Liposomes have been recognized as new delivery vehicles for peptide and oli
gonucleotide drugs, offering effective drug protection and influencing drug
distribution from the circulation to tissues. To ensure consistent formula
tion behavior and drug distribution, the amounts of free and encapsulated a
ctive pharmaceutical ingredient in the liposome formulation must be determi
ned. A simple and reliable method has been developed for the determination
of free and encapsulated oligonucleotide drugs in liposomes. Capillary elec
trophoresis in entangled polyacrylamide solution was optimized for the anal
ysis. Liposome samples were treated to release encapsulated oligonucleotide
so that total concentration could be determined, and untreated liposomes w
ere applied directly to capillary column to determine the fraction of free
oligonucleotides. Recoveries are 96-105% and relative standard deviations a
re generally 2-3%. Multiple liposome types were analyzed with satisfactory
results. (C) 2000 Elsevier Science B.V. All rights reserved.