Fyt. Sin et al., Electroporation of salmon sperm for gene transfer: Efficiency, reliability, and fate of transgene, MOL REPROD, 56(2), 2000, pp. 285-288
Uptake of exogenous DNA by electroporated salmon sperm for gene transfer is
being investigated. Our studies show that electroporated salmon sperm cell
s were more efficient and more reliable than untreated sperm in picking up
exogenous DNA and subsequently transferring the DNA into salmon embryos. In
direct evidence suggest that some of the exogenous DNA was internalized in
the sperm nuclei. The taken up DNA retained its integrity as demonstrated b
y PCR. The foreign DNA was detected in 15-month-old fish, and had a mosaic
pattern of distribution. Integration of the foreign DNA occurred infrequent
ly, and the expression of the foreign genes was poor. The potential of sper
m-mediated gene transfer as a routine protocol for mass gene transfer in sa
lmon will be dependent on the improvement of integration and expression of
the foreign gene. (C) 2000 Wiley-Liss, Inc.