Electroporation of salmon sperm for gene transfer: Efficiency, reliability, and fate of transgene

Citation
Fyt. Sin et al., Electroporation of salmon sperm for gene transfer: Efficiency, reliability, and fate of transgene, MOL REPROD, 56(2), 2000, pp. 285-288
Citations number
15
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR REPRODUCTION AND DEVELOPMENT
ISSN journal
1040452X → ACNP
Volume
56
Issue
2
Year of publication
2000
Supplement
S
Pages
285 - 288
Database
ISI
SICI code
1040-452X(200006)56:2<285:EOSSFG>2.0.ZU;2-2
Abstract
Uptake of exogenous DNA by electroporated salmon sperm for gene transfer is being investigated. Our studies show that electroporated salmon sperm cell s were more efficient and more reliable than untreated sperm in picking up exogenous DNA and subsequently transferring the DNA into salmon embryos. In direct evidence suggest that some of the exogenous DNA was internalized in the sperm nuclei. The taken up DNA retained its integrity as demonstrated b y PCR. The foreign DNA was detected in 15-month-old fish, and had a mosaic pattern of distribution. Integration of the foreign DNA occurred infrequent ly, and the expression of the foreign genes was poor. The potential of sper m-mediated gene transfer as a routine protocol for mass gene transfer in sa lmon will be dependent on the improvement of integration and expression of the foreign gene. (C) 2000 Wiley-Liss, Inc.