Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1.

Authors
Graham, KA Leithoff, J Coe, IR Mowles, D Mackey, JR Young, JD Cass, CE
Citation
Ka. Graham et al., Differential transport of cytosine-containing nucleosides by recombinant human concentrative nucleoside transporter protein hCNT1., NUCLEOS NUC, 19(1-2), 2000, pp. 415-434
Citations number
33
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
19
Issue
1-2
Year of publication
2000
Pages
415 - 434
Database
ISI
SICI code
1525-7770(2000)19:1-2<415:DTOCNB>2.0.ZU;2-0
Abstract
The transportability of cytosine-containing nucleosides by recombinant hCNT 1 was investigated in transfected mammalian cells. Apparent K-m values for hCNT1-mediated transport of uridine, cytidine and deoxycytidine were, respe ctively, 59, 140 and 150 mu M. Uridine transport was inhibited 89, 32 and 1 1%, respectively, by 500 CIM gemcitabine, cytarabine and lamivudine, demons trating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarabi ne and lamivudine are poor hCNT1 permeants.