Antibacterial activity of ozonized sunflower oil, Oleozon, against Staphylococcus aureus and Staphylococcus epidermidis.

Ozonized sunflower oil, Oleozon, has shown antimicrobial effects against se veral bacterial species. The purpose of this paper was to study in vitro ac tivity of ozonized sunflower oil, Oleozon, on Staphylococcus aureus and Sta phylococcus epidermidis, methicillin susceptible and methicillin resistant strains. Minimal Inhibitory Concentrations (MICs) and Minimal Bactericidal Concentrations (MBCs) were determined based on National Committee for Clini cal Laboratory Standards (NCCLS) guidelines. Meanwhile, kinetic studies wer e performed. Oleozon was effective against all strains, MIC90 was 9.5 mg/mL and MBC90 was 356 mg/mL. First order inactivation kinetics with respect to bacterial concentrations were found. The longest time necessary to obtain the total inactivation was 60 minutes for methicillin susceptible strains a nd 180 minutes for methicillin resistant strains.