The present study was designed to investigate which subtypes of spinal 5-HT
receptors are involved in 5-MT-induced antinociception using the mechanica
l pain test. Serotonin and various selective antagonists or agonists for 5-
HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT2A, 5-HT2C, 5-HT3 and 5-HT4) were
administered intrathecally (i.t.) in rats. The i.t. injection of 5-HT (1 m
u g) produced significant antinociceptive effects using the paw pressure te
st. Pretreatment with the 5-HT2C receptor antagonist mesulergine (1 and 10
mu g) and the 5-HT3 receptor antagonist tropisetron (1 and 10 mu g) reverse
d totally the antinociception induced by 5-HT. Furthermore, at a dose of 10
mu g, both the 5-HT2A receptor antagonist ketanserin and the 5-HT1B recept
or antagonist penbutolol, but neither the 5-HT1A receptor antagonist WAY 10
0635 nor the 5-HT4 receptor antagonist GR113808, attenuated the antinocicep
tive effect induced by 5-HT. In addition, an i.t. injection of the 5-HT3 ag
onist mCPBG induced significant antinociceptive effects whereas the 5-HT2 a
gonist DOI did not produce analgesia. These results suggest that although t
he precise degree of the involvement of spinal serotonergic 5-HT3 receptors
remains to be elucidated due to some differences in the effect of agonists
or antagonists, these receptors seem to play a role in the antinociceptive
effect of 5-HT against a mechanical acute noxious stimulus. The involvemen
t of 5-HT2C is more questionable due to the observed discrepancies between
the effects of the used agonist and antagonist. 5-HT1A and 5-HT4 receptors
do not seem to be involved. In addition, a possible functional interaction
between spinal serotonergic receptors may exist. (C) 2000 International Ass
ociation for the Study of Pain. Published by Elsevier Science B.V. All righ
ts reserved.