Maize plants of CPB2 and CPB8 hybrids were kept under water deficit for 22
d, In the CPB8 hybrid, leaf rolling initiated at the 9(th) d of water defic
it period, while in CPB2 hybrid it was at the 15(th) d, Both hybrids showed
leaf rolling initiation at the same leaf water potential, Psi(w) of -0.480
+/-0.095 MPa. At leaf rolling initiation, the leaf osmotic potential, Psi(s
) was -0.730+/-0.085 MPa in CPB8 and 0.630+/-0.110 MPa in CPB2, The leaf te
mperature and stomatal conductance were higher in CPB8 than in CPB2. Values
of leaf Psi(w), ribulose-1,5-bisphosphate carboxylase activity, chlorophyl
l content, and specific leaf area were similar in both hybrids. Phosphoenol
pyruvate carboxylase activity and protein content were lower in the CPB2 hy
brid than in CPB8, In both hybrids leaf rolling initiation was associated w
ith: (1) higher leaf temperature, with leaf rolling effect related to leaf
temperature reduction, and (2) lower leaf Psi(s), related to osmotic adjust
ment as an additional component of drought-tolerance strategy.