SEQUENCE AND EXPRESSION OF MOUSE PROTEASOME ACTIVATOR PA28 AND THE RELATED AUTOANTIGEN KI

Using reverse transcription polymerase chain reaction, we cloned and s equenced full-length mouse cDNAs for the two homologous subunits of th e proteasome activator PA28 (PA28 alpha and PA28 beta), as well as for the related protein Ki. These proteins are highly conserved among spe cies. Northern blot analysis of PA28a, PA28b, and Ki mRNA demonstrated broad tissue distribution. Although single transcripts were detected for PA28a and PA28b, two different sized transcripts were defected for mouse Ki, suggesting either alternative splicing or alternate polyade nylation sites. The levels of these transcripts increased in response to interferon-gamma (IFN-gamma) treatment in mouse H6 hepatoma cells, although PA28a and PA28b were induced to a greater extent than Ki, and the effect of IFN-gamma stimulation on Ki expression was transient. S outhern blot analysis suggests that both PA28a and PA28b are multiple- copy genes, while Ki is a single-copy gene.