Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarin
us officinalis were grown on a basal nutrient medium for 9 weeks. The regen
erants were shown by means of HPLC and mass spectrometry to contain carnosi
c acid, a diterpenoid with antioxidant and medicinal properties. Five-week-
old nodular green callus also contained carnosic acid, whereas non-green, u
ndifferentiated callus maintained in the dark did not.