Hs. Lin et al., Development of a plant regeneration system based on friable embryogenic callus in the ornamental Alstroemeria, PL CELL REP, 19(5), 2000, pp. 529-534
Stem segments of seedlings from two Alstroemeria breeding lines, cultured o
n media supplemented with 4 mg/l 2,4-dichlorophenoxyacetic acid and 0.5-1.0
mg/l 6-benzylaminopurine (BA), initiated soft callus, which became compact
after subculture on a medium with only 0.5 mg/l BA. Friable embryogenic ca
lli were initiated from compact callus on a medium supplemented with 10 mg/
l picloram. Proembryos developed from friable embryogenic calli via embryos
into plants after subculture on medium supplemented with 0.1 mg/l BA. The
proembryos formed friable embryogenic calli again after culture on medium s
upplemented with 10 mg/l picloram. The total time needed to regenerate a co
mplete plantlet from friable callus was approximately 6 months. This system
for the production of embryogenic material is considered to have valuable
applications for genetic transformation in Alstroemeria.