The action of adenosine deaminase (EC 3.5.4.4.) on adenosine and deoxyadenosine acetates: The crucial role of the 5 '-hydroxy group for the enzyme activity

Authors
Ciuffreda, P Casati, S Santaniello, E
Citation
P. Ciuffreda et al., The action of adenosine deaminase (EC 3.5.4.4.) on adenosine and deoxyadenosine acetates: The crucial role of the 5 '-hydroxy group for the enzyme activity, TETRAHEDRON, 56(20), 2000, pp. 3239-3243
Citations number
22
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON
ISSN journal
00404020 → ACNP
Volume
56
Issue
20
Year of publication
2000
Pages
3239 - 3243
Database
ISI
SICI code
0040-4020(20000512)56:20<3239:TAOAD(>2.0.ZU;2-R
Abstract
From adenosine 1,2'-deoxyadenosine 3 and 3'-deoxyadenosine 5 all the acetat es were prepared by lipase-catalyzed reactions. Only the acetates with free 5'-hydroxy group were deaminated by adenosine deaminase (ADA), confirming the crucial role of 5'-OH for the enzyme activity. (C) 2000 Elsevier Scienc e Ltd. All rights reserved.