R. Sodian et al., Fabrication of a trileaflet heart valve scaffold from a polyhydroxyalkanoate biopolyester for use in tissue engineering, TISSUE ENG, 6(2), 2000, pp. 183-188
Previously, we reported the implantation of a single tissue engineered leaf
let in the posterior position of the pulmonary valve in a lamb model. The m
ajor problems with this leaflet replacement were the scaffold's inherent st
iffness, thickness, and nonpliability. We have now created a scaffold for a
trileaflet heart valve using a thermoplastic polyester. In this experiment
, we show the suitability of this material in the production of a biodegrad
able, biocompatible scaffold for tissue engineered heart valves. A heart va
lve scaffold was constructed from a thermoplastic elastomer. The elastomer
belongs to a class of biodegradable, biocompatible polyesters known as poly
hydroxyalkanoates (PHAs) and is produced by fermentation (Metabolix Inc., C
ambridge, MA). It was modified by a salt leaching technique to create a por
ous, three-dimensional structure, suitable for tissue engineering. The tril
eaflet heart valve scaffold consisted of a cylindrical stent (1 mm x 15 mm
x 20 mm I.D.) containing three valve leaflets. The leaflets were formed fro
m a single piece of PHA (0.3 mm thick), and were attached to the outside of
the stent by thermal processing techniques, which required no suturing. Af
ter fabrication, the heart valve construct was allowed to crystallize (4 de
grees C for 24 h), and salt particles mere leached into doubly distilled wa
ter over a period of 5 days to yield pore sizes ranging from 80 to 200 micr
ons. Ten heart valve scaffolds were fabricated and seeded with vascular cel
ls from an ovine carotid artery. After 4 days of incubation, the constructs
were examined by scanning electron microscopy. The heart valve scaffold wa
s tested in a pulsatile flow bioreactor and it was noted that the leaflets
opened and closed. Cells attached to the polymer and formed a confluent lay
er after incubation. One advantage of this material is the ability to mold
a complete trileaflet heart valve scaffold without the need for suturing le
aflets to the conduit. Second advantage is the use of only one polymer mate
rial (PHA) as opposed to hybridized polymer scaffolds. Furthermore, the mec
hanical properties of PHA, such as elasticity and mechanical strength, exce
ed those of the previously utilized material. This experiment shows that PH
As can be used to fabricate a three-dimensional, biodegradable heart valve
scaffold.