3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation-reduction process: anintegrated approach combining EXAFS and crystallography

Authors
Cheung, KC Strange, RW Hasnain, SS
Citation
Kc. Cheung et al., 3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation-reduction process: anintegrated approach combining EXAFS and crystallography, ACT CRYST D, 56, 2000, pp. 697-704
Citations number
44
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
6
Pages
697 - 704
Database
ISI
SICI code
0907-4449(200006)56:<697:3EROTC>2.0.ZU;2-E
Abstract
Three-dimensional information is obtained for the Cu site in azurin at very high resolution by combining high-resolution crystallographic structures a nd EXAFS data for the oxidized and reduced form of the protein. This combin ed approach has allowed us to define the subtle structural changes (<0.1 An gstrom) which take place at the Cu site during a single-electron redox proc ess.