The structure of human aldose reductase bound to the inhibitor IDD384

Citation
V. Calderone et al., The structure of human aldose reductase bound to the inhibitor IDD384, ACT CRYST D, 56, 2000, pp. 536-540
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
5
Pages
536 - 540
Database
ISI
SICI code
0907-4449(200005)56:<536:TSOHAR>2.0.ZU;2-2
Abstract
The crystallographic structure of the complex between human aldose reductas e (AR2) and one of its inhibitors, IDD384, has been solved at 1.7 Angstrom resolution from crystals obtained at pH 5.0. This structure shows that the binding of the inhibitor's hydrophilic head to the catalytic residues Tyr48 and His110 differs from that found previously with porcine AR2. The differ ence is attributed to a change in the protonation state of the inhibitor (p K(a) = 4.52) when soaked with crystals of human (at pH 5.0) or pig lens AR2 (at pH 6.2). This work demonstrates how strongly the detailed binding of t he inhibitor's polar head depends on its protonation state.