Yx. Ding et al., Lead promotes hydroxyl radical generation and lipid peroxidation in cultured aortic endothelial cells, AM J HYPERT, 13(5), 2000, pp. 552-555
Early studies by our group have shown that lead-induced hypertension (HTN)
is closely related to enhanced activity of reactive oxygen species (ROS). I
n addition, we have found indirect evidence that hydroxyl radical may be th
e most likely culprit in lead-exposed animals. In the present study, rat ao
rtic endothelial cells were incubated in the presence of 0, 0.01, 0.1, 0.5,
and 1.0 ppm lead acetate for 1, 24, and 48 h. At the conclusion of the inc
ubation period cells were harvested and the media were collected. Lipid per
oxidation products were measured as malondialdehyde-thiobarbituric acid (MD
A-TBA) in the medium and hydroxyl radical was measured as 2,3-dihydroxybenz
oic acid (2,3 DHBA) in the cells. After exposure to lead for 48 h, MDA-TBA
generation and 2,3 DHBA formation were significantly increased. These data
clearly demonstrate that lead exposure promotes hydroxyl radical generation
and induces oxidative stress in isolated endothelial cells, mimicking the
effects observed in lead-exposed animals. Enhanced inactivation of endothel
ium-derived nitric oxide by locally produced oxygen free radicals could con
tribute to endothelial dysfunction and HTN in lead-exposed animals. (C) 200
0 American Journal of Hypertension, Ltd.