The potential of fluorescence polarization for fluorescence mixture resolut
ion was assessed and compared with liquid chromatography (LC). Careful sele
ction of excitation wavelengths and the use of an appropriate viscous mediu
m (glycerine) allows significant differences in polarization to be obtained
. For two component mixtures, information about fluorescence polarization a
nd the total intensity of the samples is sufficient to calculate the relati
ve contributions when the polarization of the two pure components are known
. The results obtained for benomyl and carbaryl show detection limits (K S-
B/m) of 11.4 ng ml(-1) and 1.2 ng ml(-1), mid-range relative standard devia
tions of 0.8% and 0.9% n=3 and recoveries between 104 and 113% and 91 and 1
07%, respectively. (C) 2000 Elsevier Science B.V. All rights reserved.