A 2-aminopyridine molecularly imprinted polymer surrogate micro-column forselective solid phase extraction and determination of 4-aminopyridine

Poly(methacrylic acid-ethylene glycol dimethacrylate) was prepared using 2- aminopyridine as the imprinting molecule. This molecularly imprinted polyme r (MIP) was ground and packed into a micro-column for selective solid phase extraction (SPE) of 2-aminopyridine from 20 mu l of sample solution. Non-s pecific adsorption was also confirmed for a structural analogue. Interestin gly one of the isomers, 4-aminopyridine, bound most strongly to the MIP. Th e implication of resonance and basicity of this isomer molecule can be used to explain its strong binding with the self-assembled functional methacryl ic acid (MAA) monomer. The monomer template complexion process was evaluate d by Scatchard plots to determine a binding constant. The binding constant value is important for predicting the selectivity of a new MIP. After optim ization of the molecular recognition process, a molecularly imprinted solid phase extraction-differential pulsed elution (MISPE-DPE) method was develo ped for the selective determination of 4-aminopyridine in serum with an ana lysis time of less than 3 min using a 2-aminopyridine micro-column for surr ogate binding. The concentration detection limit was 0.5 mu g/ml, which cor responded to an absolute detection limit of 10 ng. A larger sample volume o f 845 mu l afforded a better concentration detection limit of 52 ng/ml. (C) 2000 Elsevier Science B.V. All rights reserved.