Autologous cultured fibroblasts: A protein repair system

Cultured autologous fibroblasts create a living injectable system that has been utilized effectively to treat rhytids, depressed scars, subcutaneous a trophy, acne irregularities, and laser wounds. Autologous cultured fibrobla sts (Isolagen) as a protein repair system is produced from a 3-mm cutaneous punch biopsy. Skin biopsies are initiated in an in vitro tissue culture sy stem. The cells are expanded to produce large quantities of fibroblasts and extracellular matrix totaling 1.0 to 1.5 mi. This cellular system is injec ted into the patient after 8 weeks of cellular expansion. Each area of trea tment is injected once every 2 weeks for three injections. A total of 1,450 patients in the United States and Europe have been treated at designated c enters. A total of 4,800 injections were given. At the University of Medici ne and Dentistry of New Jersey and Hackensack University Medical Center, 94 patients were treated from 1995 through 1999. Long-term follow-up ranged f rom 36 to 48 months. A subjective patient satisfaction survey showed 92% of the patients were satisfied with the grade of correction. A long-term foll ow-up survey revealed continuing improvement beyond the initial correction in 70% of patients. Results from other designated centers correlated with t he authors' findings. Cultured autologous fibroblasts (Isolagen) appears to be a living, cellular, dynamic filler system capable of immediate correcti on and continued repair of dermal and superficial subcutaneous deficiencies .