Cloning and characterization of an epoxide hydrolase-encoding gene from Rhodotorula glutinis

Authors
Visser, H Vreugdenhil, S de Bont, JAM Verdoes, JC
Citation
H. Visser et al., Cloning and characterization of an epoxide hydrolase-encoding gene from Rhodotorula glutinis, APPL MICR B, 53(4), 2000, pp. 415-419
Citations number
19
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
01757598 → ACNP
Volume
53
Issue
4
Year of publication
2000
Pages
415 - 419
Database
ISI
SICI code
0175-7598(200004)53:4<415:CACOAE>2.0.ZU;2-C
Abstract
We cloned and characterized the epoxide hydrolase gene, EPH1, from Rhodotor ula glutinis. The EPH1 open reading frame of 1230 bp was interrupted by nin e introns and encoded a polypeptide of 409 amino acids with a calculated mo lecular mass of 46.3 kDa. The amino acid sequence was similar to that of mi crosomal epoxide hydrolase, which suggests that the epoxide hydrolase of R. glutinis also belongs to the alp hydrolase fold family. EPH1 cDNA was expr essed in Escherichia coli and resting cells showed a specific activity of 2 00 nmol min(-1) (mg protein)(-1) towards 1,2-epoxyhexane.