Restriction endonuclease analysis of the genome of two Italian caprine herpesvirus 1 strains

Citation
A. Pratelli et al., Restriction endonuclease analysis of the genome of two Italian caprine herpesvirus 1 strains, ARCH VIROL, 145(4), 2000, pp. 845-851
Citations number
26
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
145
Issue
4
Year of publication
2000
Pages
845 - 851
Database
ISI
SICI code
0304-8608(2000)145:4<845:REAOTG>2.0.ZU;2-P
Abstract
Two caprine herpesvirus (CpHV.1) strains isolated in two goat flocks in sou thern Italy were compared with E/CH and McK/US reference CpHV.1 strains by restriction endonuclease analysis. BamHI, KpnI, NotI and PstI restriction e nzymes were used. With these enzymes McK/US strain was clearly differentiat ed from the others. KpnI and NotI digestion resulted in unique patterns for all four strains. Restriction enzyme analysis might be a useful method to distinguish CpHV.1 isolated from animals and provide a basis for further ep idemiological studies of the CpHV.1 infection.