U. Machida et al., The effect of granulocyte colony-stimulating factor administration in healthy donors before bone marrow harvesting, BR J HAEM, 108(4), 2000, pp. 747-753
To investigate whether granulocyte colony-stimulating factor (G-CSF) admini
stration to donors before harvest may lighten the burden imposed on them an
d accelerate the bone marrow (BM) recovery, we administered 2 mu g/kg/d of
G-CSF for five consecutive days before the marrow harvest, All of the donor
s tolerated the G-CSF administration well without severe adverse events, Af
ter 5 d of G-CSF treatment, CD34(+) cells and granulocyte-macrophage colony
-forming units (GM-CFU) in the donors' BM exceeded baseline values by 4.2-f
old (range 0.71-316) and 1.6-fold (0.28-118) respectively. The concentratio
n of total nucleated cells (x 10(7)/ml) in the graft increased from 1.61 (0
.95-3.23) to 2.44 (1.27-4.01). Although we collected 1020 ml of BM and obta
ined 1.50 x 10(10) nucleated cells from unprimed donors, 940 ml of BM were
sufficient to obtain 2.14 x 10(10) nucleated cells from primed donors. Howe
ver, G-CSF-primed BM did not shorten the time to trilineage engraftment and
the duration of hospitalization compared with unprimed BM, although primed
BM contained more CD34(+) cells than baseline values. We consider that the
advantages of BM priming are not the acceleration of BM recovery but rathe
r the reduction of blood loss during BM harvesting.