The effect of granulocyte colony-stimulating factor administration in healthy donors before bone marrow harvesting

Citation
U. Machida et al., The effect of granulocyte colony-stimulating factor administration in healthy donors before bone marrow harvesting, BR J HAEM, 108(4), 2000, pp. 747-753
Citations number
25
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
108
Issue
4
Year of publication
2000
Pages
747 - 753
Database
ISI
SICI code
0007-1048(200003)108:4<747:TEOGCF>2.0.ZU;2-5
Abstract
To investigate whether granulocyte colony-stimulating factor (G-CSF) admini stration to donors before harvest may lighten the burden imposed on them an d accelerate the bone marrow (BM) recovery, we administered 2 mu g/kg/d of G-CSF for five consecutive days before the marrow harvest, All of the donor s tolerated the G-CSF administration well without severe adverse events, Af ter 5 d of G-CSF treatment, CD34(+) cells and granulocyte-macrophage colony -forming units (GM-CFU) in the donors' BM exceeded baseline values by 4.2-f old (range 0.71-316) and 1.6-fold (0.28-118) respectively. The concentratio n of total nucleated cells (x 10(7)/ml) in the graft increased from 1.61 (0 .95-3.23) to 2.44 (1.27-4.01). Although we collected 1020 ml of BM and obta ined 1.50 x 10(10) nucleated cells from unprimed donors, 940 ml of BM were sufficient to obtain 2.14 x 10(10) nucleated cells from primed donors. Howe ver, G-CSF-primed BM did not shorten the time to trilineage engraftment and the duration of hospitalization compared with unprimed BM, although primed BM contained more CD34(+) cells than baseline values. We consider that the advantages of BM priming are not the acceleration of BM recovery but rathe r the reduction of blood loss during BM harvesting.