Characterization of chromosomal aberrations in human gastric carcinoma cell lines using chromosome painting

Using chromosome painting, a study of chromosomal abnormalities was perform ed in six gastric carcinoma cell lines (SNU-484, 601, 620, 638, 668, 719) f rom Korean patients. Each carcinoma cell line had unique modal karyotypic c haracteristics and showed a variable number of numerical and structural clo nal cytogenetic aberrations. SNU-484, SNU-620, and SNU-668 had near-triploi dy; SNU-601, SNU-638, and SNU-719 had near-diploidy. The origins of the mar ker chromosomes of these cell lines it ere identified by fluorescence in si tu hybridization with constructed painting probes. In all of six cell lines , rearrangement of chromosome 17 resulting in partial deletion of 17p (and/ or partial duplication of 17q) was found. The most frequent marker ri as a par tial gain of chromosome 7 with the breakpoints on 7q22 and 7q31. The no nrandom rearrangements of chromosomes Mere also determined on 1q32, 5q11-q2 2, 8q, 14q22, 14q34, and 15q15; suggesting that they may be the candidate r egions for the isolation of the genes related to gastric cancer. (C) 2000 E lsevier Science Inc. All rights reserved.