Phylogenetic analysis of mitochondrial and nuclear sequences supports inclusion of Acantholingua ohridana in the genus Salmo

The mitochondrial cytochrome b gene was sequenced from Acantholingua ohrida na from Lake Ohrid, Macedonia, and Salmo salar; and these sequences were co mpared to those previously obtained from S. trutta and five other salmonid species. In addition, a nuclear sequence, the first internal transcribed sp acer (ITS1) of the ribosomal DNA, was obtained from A. ohridana, S. salar, and S. trutta and compared with sequences obtained from the same five salmo nid species. Combination of these data with nuclear GH2C intron sequences f or the same taxa yielded a combined dataset of approximately 2100 aligned b p for eight species. Phylogenetic analysis of all datasets supported the in clusion of A. ohridana in Salmo and a sister relationship between A. oridan a and S. trutta.