Determination of lipoprotein(a) concentrations and apolipoprotein(a) molecular weights in diabetic patients

Citation
A. Ribault et al., Determination of lipoprotein(a) concentrations and apolipoprotein(a) molecular weights in diabetic patients, DIABETE MET, 26(2), 2000, pp. 107-112
Citations number
22
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
DIABETES & METABOLISM
ISSN journal
12623636 → ACNP
Volume
26
Issue
2
Year of publication
2000
Pages
107 - 112
Database
ISI
SICI code
1262-3636(200004)26:2<107:DOLCAA>2.0.ZU;2-X
Abstract
Lipoprotein(a) (Lp(a)) with atherogenic and thrombotic properties has been frequently studied in diabetes, because a high cardiovascular risk has been reported both in type 1 and type 2 diabetes. Few studies have considered g enetic factors, especially the isoforms of apolipoprotein(a). The aim of th is work is to determine the distribution of apo(a) phenotypes in the serum of 148 diabetic patients (59 type 1, 89 type 2) with or without vascular co mplications. Apo(a) phenotypes are determined using 4-15% sodium dodecyl su lfate polyacrylamide gel electrophoresis followed by immunoblotting (PhastS ystem - Pharmacia). An inverse relationship is observed between Lp(a) serum concentration and the apparent molecular mass of apo(a) isoforms: type 1 r = - 0.61, p < 0.01; type 2 r = - 0.55, p < 0.01. The frequency of apo(a) i soforms is significantly different between type 1 and type 2 diabetes melli tus. A higher prevalence of isoforms of low molecular weight was observed i n the type 2 diabetic population.