Hj. Zambarakji et al., Reproducibility of volumetric macular measurements in diabetic patients with the Heidelberg Retina Tomograph, DOC OPHTHAL, 97(3-4), 1999, pp. 349-360
Aims: To quantify diabetic macular edema by confocal scanning laser ophthal
moscopy and assess its usefulness by determining the reproducibility of top
ographic measurements at the macula. Methods: The volumes above reference p
lane bound by a 2 mm diameter circle centred on the fovea were measured by
two observers. The reference plane was adjusted to the lowest point of the
height variation of the contour line. The reproducibility of this technique
was assessed in 20 eyes of 20 diabetic patients of which 8 eyes had macula
r edema. Three HRT scans of each eye were obtained. The measurements of vol
ume above reference plane of each scan were repeated three times. Results:
For all diabetic eyes, the intra scan coefficients of variability measured
14.71% to 21.21%, the inter scan coefficient of variability was 30.46%. The
average standard deviations were 0.053 mm(3) for one examination per scan,
0.047 mm(3) for two examinations per scan and 0.044 mm(3) for three examin
ations per scan. Linear regression demonstrated an increase in standard dev
iation with greater volumetric measurements (p < 0.001). We found good corr
elation (r = 0.959, p < 0.001) and strong agreement between the two observe
r's findings for all 20 eyes. For the 8 eyes with macular edema, the coeffi
cients of variability were similar to those calculated for all 20 eyes. The
average SD for one examination per scan were 0.078 mm(3), 0.069 mm(3) for
two examinations per scan and 0.062 mm(3) for three examinations per scan.
We found good correlation (r = 0.945, p < 0.001) and strong agreement betwe
en the two observer's findings in eyes with edema. Conclusion: The reproduc
ibility of this technique has been demonstrated in diabetic eyes. This may
have useful clinical applications for the quantification of diabetic macula
r edema and monitoring of laser therapy.