A novel sensitive colorimetric assay for visual detection of solid-phase bound amines

Authors
Madder, A Farcy, N Hosten, NGC De Muynck, H De Clercq, PJ Barry, J Davis, AP
Citation
A. Madder et al., A novel sensitive colorimetric assay for visual detection of solid-phase bound amines, EUR J ORG C, (11), 1999, pp. 2787-2791
Citations number
25
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
ISSN journal
1434193X → ACNP
Issue
11
Year of publication
1999
Pages
2787 - 2791
Database
ISI
SICI code
1434-193X(199911):11<2787:ANSCAF>2.0.ZU;2-0
Abstract
A new simple and efficient method for the detection of incomplete coupling reactions during solid-phase peptide synthesis is decribed. Using p-nitroph enyl ester 1 (NF31), free amino groups can be visually detected on the resi n by direct coloring of the beads. A specific feature of the assay resides in the possibility of detection of sterically hindered primary amines.