Neuronal connections in the medial geniculate body of the guinea-pig

The spontaneous and evoked activities of individual pairs of single units w ere recorded simultaneously with the same microelectrode in the medial geni culate body (MGB) of ketamine-xylazine-anaesthetised guinea-pigs. Cross-cor relograms (CCGs) of spike train pairs were computed and divided on the basi s of correlation peak shape into four classes [a unilateral narrow (UN) pea k, a centrally positioned wide (CW) peak, a complex peak and no significant peak] interpreted in terms of the functional connection between neighbouri ng neurones. The shift predictor procedure was applied with the aim of remo ving the effect of the stimulus on the final CCG shape. The occurrence of c orrelation peak types and the distribution of correlation coefficients were found to be similar for the spontaneous activity during silent periods fol lowing acoustical stimulation and for the long-lasting recording of spontan eous activity. CCGs in 38% of pairs computed during silent interstimulus in tervals contained a UN peak, suggesting a monosynaptic excitatory connectio n. Almost 20% of all pairs expressed a CCG shape typical for a common input , i.e. a CW peak. In 5% of cases multiple, so-called complex peaks, were fo und. About 20% of the CCGs contained no significant correlation peak in the interstimulus period, which is typical for a very weak or absent functiona l connection between recorded neurones. No inhibitory interaction (groove i n the CCGs) between recorded pairs was observed. The distribution of correl ation peak shapes was similar when calculated during acoustical stimulation and during silent interstimulus intervals. CCGs computed during presentati on of four acoustical stimuli (pure tone bursts, noise bursts, natural call whistle and artificially inverted whistle) showed most frequently a UN pea k (28-37%) followed by CCGs with no significant peak (18-28%) and with a UN /CW peak (14-23%). On average, the occurrence of UN peaks tended to be less frequent during stimulus presentation than in silent conditions, but the d ifference was not statistically significant. The most frequent occurrence o f clear UN peaks was found in the medial part of the MGB (from 52-64% of pa irs depending on the type of acoustical stimulus), while the least was obse rved in the ventral part of the MGB (12-22%). In contrast, CW peaks were mo st frequently expressed in pairs located in the ventral part of the MGB (18 -33%), while neuronal pairs in the medial part revealed a very low occurren ce of CW peaks (0-7%). The occurrence of independently firing neurones was lowest in the medial part of the MGB (8-20% of pairs) in comparison with th e ventral (31-39%) and dorsal (12-41%) parts. In 20% of pairs acoustical st imulation produced a change in the type of correlation peak present during spontaneous activity. Most frequently, a CW peak (shared input) changed to a flat CCG, which represents independently firing neurones. In some pairs h igher connection strengths (as expressed by the value of the correlation co efficient) were found for silent interstimulus intervals than for acoustica l stimulation. This suggests that in the MGB the stimulus may desynchronise the spontaneous activity of simultaneously firing units in neuronal pairs.