In this EU (DG XII) funded project, a Core human study was conducted in Ire
land, Northern Ireland, Spain, France and The Netherlands. Oxidative and an
tioxidant status, vegetable and fruit consumption, and carotenoid intake of
volunteers from different countries was compared. Response to increased ca
rotenoid intake was determined using a range of measurements. Attention was
paid to whether the antioxidant capability of beta-carotene,lutein and lyc
opene demonstrated in vitro, was apparent in relation to increased oxidatio
n resistance of low-density-lipoprotein (LDL). Other (Complementary) studie
s were undertaken and included determination of: protective effects of caro
tenoid-rich foods against LDL and DNA oxidative damage; carotenoid absorbab
ility; barriers to increased vegetable consumption; and carotenoid content
of fruits and vegetables frequently consumed in Europe. Our results demonst
rated that carotenoid supplementation did not increase LDL oxidation resist
ance. However, increased consumption of carotenoid-rich fruits and vegetabl
es did increase LDL oxidation resistance, and higher plasma concentration o
f total and specific carotenoids (pre-supplementation) was associated with
lower DNA damage and higher repair activity. (C) 2000 Published by Elsevier
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