The novel G-protein coupled receptor SALPR shares sequence similarity withsomatostatin and angiotensin receptors

A cDNA encoding a novel G-protein coupled receptor (GPCP) was isolated from a human cerebral cortex cDNA library by low stringency hybridization scree ning, This putative seven-transmembrane domain receptor of 469 amino acids was designated SALPR (Somatostatin- and Angiotensin- Like Peptide Receptor) . SALPR shares the highest amount of amino acid similarity with the somatos tatin (35% with SSTR5) and angiotensin receptors (31% with AT1). Reverse tr anscription-polymerase chain reaction (RT-PCR) analysis revealed that the S ALPR mRNA is predominantly expressed in human brain regions, particularly t he substantia nigra and pituitary, although the mRNA can also be detected i n the peripheral tissues, albeit at low levels. Chromosomal mapping by radi ation hybrid analysis localized the human SALPR gene to the chromosome 5p15 .1-5p14. Transient expression of SALPR in COS-l cells did not produce any b inding sites for somatostatin or angiotensin II, indicating the necessity f or further study to discover its ligand and physiological significance. (C) 2000 Elsevier Science B.V. All rights reserved.