M. Matsumoto et al., The novel G-protein coupled receptor SALPR shares sequence similarity withsomatostatin and angiotensin receptors, GENE, 248(1-2), 2000, pp. 183-189
A cDNA encoding a novel G-protein coupled receptor (GPCP) was isolated from
a human cerebral cortex cDNA library by low stringency hybridization scree
ning, This putative seven-transmembrane domain receptor of 469 amino acids
was designated SALPR (Somatostatin- and Angiotensin- Like Peptide Receptor)
. SALPR shares the highest amount of amino acid similarity with the somatos
tatin (35% with SSTR5) and angiotensin receptors (31% with AT1). Reverse tr
anscription-polymerase chain reaction (RT-PCR) analysis revealed that the S
ALPR mRNA is predominantly expressed in human brain regions, particularly t
he substantia nigra and pituitary, although the mRNA can also be detected i
n the peripheral tissues, albeit at low levels. Chromosomal mapping by radi
ation hybrid analysis localized the human SALPR gene to the chromosome 5p15
.1-5p14. Transient expression of SALPR in COS-l cells did not produce any b
inding sites for somatostatin or angiotensin II, indicating the necessity f
or further study to discover its ligand and physiological significance. (C)
2000 Elsevier Science B.V. All rights reserved.