Electrically mediated plasmid DNA delivery to hepatocellular carcinomas invivo

Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. in vivo electr oporation has been used to enhance delivery of chemotherapeutic agents to t umors in both animal and human studies. Recently, this delivery technique h as been extended to large molecules such as plasmid DNA. Here, the successf ul delivery of plasmids encoding reporter genes to rat hepatocellular carci nomas by in vivo electroporation is demonstrated.