Efficiency of transgenic T cell generation from gene-marked cultured humanCD34(+) cord blood cells is determined by their maturity and the cytokinespresent in the culture medium

Success of gene therapy for diseases affecting the T cell lineage depends o n the thymic repopulation by genetically engineered hematopoietic progenito r cells (HPC). Although it has been shown that retrovirally transduced HPC can repopulate the thymus, little information is available on the effect of the culture protocol. Moreover, for expansion of the number of HPC, cytoki ne supplemented culture is needed. Here, we transduced purified human umbil ical cord blood (CB) CD34(+) cells in cultures supplemented with various co mbinations of the cytokines thrombopoietin (TPO), stem cell factor (SCF), f lt3/flk-2 ligand (FL), interleukin-3 (IL-3) and IL-6, and investigated thym us-repopulating ability of gene-marked HPC in vitro. Irrespective of the cy tokine cocktail used, transduced CD34(+)CD38(-) CB cells, expressing the ma rker green fluorescent protein (GFP) encoded by the MFG-GFP retrovirus, hav e both superior proliferative and thymus-repopulating potential compared wi th transduced CD34(+)CD38(+) CB cells. Effectively transduced GFP(+)CD34(+) CD38(-) HPC, cultured for 3 or 17 days, more readily generated T cells than GFP HPC from the same culture. The reverse was true in the case of CD34(+) CD38(+) HPC cultures. Finally, our results indicate that the number of GFP( +) cell progenitors actually increased during culture of CD34(+)CD38(-) HPC , in a magnitude that is determined by the cytokine cocktail used during cu lture.