C. Cloix et al., Analysis of 5S rDNA arrays in Arabidopsis thaliana: Physical mapping and chromosome-specific polymorphisms, GENOME RES, 10(5), 2000, pp. 679-690
A physical map of a pericentromeric region of chromosome 5 containing a 5S
rDNA locus and spanning -1000 kb was established using the CIC YAC clones.
Three 5S rDNA arrays were resolved in this YAC contig by PFGE analysis and
we have mapped different types of sequences between these three blocks. 5S
rDNA units from each of these three arrays of chromosome 5, and from chromo
somes 3 and 4, were isolated by PCR. A total of 38 new DNA sequences were o
btained. Two types of 5S rDNA repeated units exist: the major variant with
0.5-kb repeats and one with short repeats (251 bp) only detected on YAC 11A
3 from chromosome 3. Although the 38 sequences displayed noticeable heterog
eneity, we were able to group them according to their 5S array origin. The
presence of 5S array-specific variants was confirmed with the restriction p
olymorphism study of all the YACs carrying 5S units.