Nuclear accumulation of p53 protein and apoptosis induced by various anticancer agents, u.v.-irradiation and heat shock in primary normal human skin fibroblasts

Relationship between nuclear accumulation of p53 protein and apoptosis indu ced by both DNA damaging agents (ultraviolet-irradiation and anticancer age nts) and heat shock in normal human skin fibroblasts was investigated. Nucl ear accumulation of p53 protein and apoptosis were induced kldose-dependent ly by u.v.-irradiation and anticancer agents. Regarding heat shock treatmen t, intensive nuclear accumulation of p53 protein was induced by treatment a t 43 degrees C for 45 min, but, apoptotic cells were never detected. These results indicated that accumulation of p53 protein and apoptosis induced by DNA damaging agents were considered to have a close relationship and the f unctions of induced p53 protein are different depending on the stimulating factor.