Microsatellite instability of dinucleotide tandem repeat sequences is higher than trinucleotide, tetranucleotide and pentanucleotide repeat sequencesin prostate cancer
G. Perinchery et al., Microsatellite instability of dinucleotide tandem repeat sequences is higher than trinucleotide, tetranucleotide and pentanucleotide repeat sequencesin prostate cancer, INT J ONCOL, 16(6), 2000, pp. 1203-1209
In order to investigate whether the change in length of simple repetitive g
enomic sequences (microsatellite instability) is associated with prostate c
ancer, we analyzed 40 prostate cancer samples with 44 microsatellite loci m
arkers on chromosomes 1, 3, 5, 6, 8, 9, 11, 13, 16, 17 and X. DNA was extra
cted from normal and tumor cells of 40 microdissected cancer samples, ampli
fied by PCR and analyzed for microsatellite instability using 44 primers fo
r dinucleotide, trinucleotide, tetranucleotide and pentanucleotide repeat s
equences. The results of this study demonstrate that 45% of the prostate ca
ncer specimens (18 out of 40) showed microsatellite instability (MSI) at a
minimum of one locus using dinucleotide repeat sequences. Two out of 40 sam
ples (5%) showed MSI at a minimum of one locus using three different trinuc
leotide repeat primers (AR, SR and TBP). Ten out of 40 (25%) samples showed
MSI at a minimum of one locus using five different tetranucleotide repeat
primers (HPRT1, HPRTII, MYCL1, RE, REN). There were no MSI observed in samp
les using pentanucleotide repeat sequences. There were no MSI in benign pro
static hyperplasia samples (25 samples). These experiments suggest that the
microsatellite instability of dinucleotide tandem repeat sequences is much
higher than trinucleotide, tetranucleotide and pentanucleotide repeat sequ
ences in prostate cancer. The MSI with different lengths of nucleotide repe
at sequences did not correlate with the stage and grades of prostate cancer
.