Secretion of the Caulobacter crescentus S-layer protein: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins

Authors
Bingle, WH Nomellini, JF Smit, J
Citation
Wh. Bingle et al., Secretion of the Caulobacter crescentus S-layer protein: Further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins, J BACT, 182(11), 2000, pp. 3298-3301
Citations number
28
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
182
Issue
11
Year of publication
2000
Pages
3298 - 3301
Database
ISI
SICI code
0021-9193(200006)182:11<3298:SOTCCS>2.0.ZU;2-B
Abstract
The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) w as localized to the C-terminal 82 amino acids of the molecule. Protein yiel d studies showed that 336 or 242 C-terminal residues of RsaA mediated secre tion of >50 mg of a cellulase passenger protein per liter to the culture fl uids.