SCREENING FOR TP53 MUTATIONS IN PATIENTS AND TUMORS FROM 109 SWEDISH BREAST-CANCER FAMILIES

To estimate the prevalence of TP53 mutations in familial family brest cancer, constant denaturant gel electrophoresis (CDGE) was used to scr een exons 5-8 of the TP53 gene for germline mutations. Genomic DNA fro m 128 brest cancer patients belonging to 109 families with familial ca ncer were screened. No germline mutations were found in any ct the pat ients. We also studied TP53 mutations in tumour DNA from 51 of the sam e individuals and found mutations in 14%. This is similar to what has been reported in sporadic breast cancer.