Optimization of ion-exchange displacement separations II. Comparison of displacement separations on various ion-exchange resins

A variety of stationary-phase materials are currently available for the chr omatographic purification of biomolecules. However, the effect of various r esin characteristics on the performance of displacement chromatography has not been studied in depth. In part I, a novel iterative scheme was presente d for the rapid optimization of displacement separations in ion-exchange sy stems. In this article, the optimization scheme is employed to identify the optimum operating conditions for displacement separations on various ion-e xchange resin materials. In addition, the effect of different classes of se paration problems (e.g., diverging, converging or parallel affinity lines) on the performance of displacement separations is also presented. The solid film linear driving force model is employed in concert with the Steric Mas s Action isotherm to describe the chromatographic behavior in these systems . The results presented in this article provide insight into the effects of resin capacity and efficiency as well. as the type of separation problem o n the performance of various ion-exchange displacement systems. (C) 2000 El sevier Science B.V. All rights reserved.