An array of 10 wild Brassica species with chromosome number 2n = 18 re
presented by 34-populations was analyzed For genome similarity using g
enomic and cDNA clones. Species studied included B. bourgeaui (Webb) O
. Kuntze, B. cretica Lam., B. hilarionis G.E. Post, B. incana Ten., B.
insularis Moris, B. macrocarpa (Guss.) Caruel, B, montana Pourret, B.
oleracea L., B, rupestris Rafin., and B, villosa Biv. The RFLP data w
ere used to calculate similarities between populations that were subse
quently treated in a cluster analysis. Most populations of a species w
ere grouped together and were separate from populations of other speci
es. The previously identified B. incana - B. rupestris - B. villosa co
mplex was verified, and genetic similarity between the species B, mont
ana and B. oleracea was evident. An interesting association between B.
insularis and B. macrocarpa was observed, The UPGMA analysis showed t
hat the species tended to cluster according to geographic region: B. c
retica and B. hilarionis comprise a cluster that could be called Easte
rn Mediterranean; B. oleracea, B, bourgeaui, and B, montana define an
Atlantic - Western Mediterranean cluster; B. incana, B. rupestris, and
B. villosa form an Italian group; and a B. insularis - B. macrocarpa
association may be called Central Mediterranean.