Population studies and validation of paternity determinations by six microsatellite loci

A single locus system of 6 microsatellite markers was evaluated for paterni ty testing. A nonradioactive method based on peroxidase labeling of a DNA p robe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134-1698 chromosomes. T he number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively , and the allele frequency varied from 0.001 to 0.317. The genotype of 87 f amilies, consisting of mother, father, and child was determined. The probab ility that a random individual will give a positive paternity was evaluated . We conclude that the markers can be reliably typed and give sufficient an d reliable information for paternity testing.