A. Meissner et Ow. Sorensen, Suppression of diagonal peaks in three-dimensional protein NMR TROSY-type HCCH correlation experiments, J MAGN RES, 144(1), 2000, pp. 171-174
A novel method for suppression of C-13-C-13 diagonal peaks without sensitiv
ity loss in three-dimensional HCCH TROSY-type NMR correlation experiments i
nvolving aromatic side chains in proteins (Pervushin et al., J, Am. Chem. S
ec. 120, 6394-6400 (1998)) is presented. The key element is a spin-state-se
lective filter in the C-13-C-13 mixing sequence with the dual effect of sel
ecting the TROSY resonance in the preceding evolution period and interchang
ing TROSY and anti-TROSY resonances. The cross peaks are invariant to this
filter but diagonal peak coherence gets concentrated on the anti-TROSY tran
sition so that it can be eliminated by a C-13 --> H-1 TROSY transfer elemen
t. The new method is demonstrated using a C-13, N-15-labeled protein sample
, RAP 18-112 (N-terminal domain of alpha(2)-macroglobulin receptor associat
ed protein), at 750 MHz. (C) 2000 Academic Press.