HPLC analysis and optimization of enzymatic synthesis of 4 '-O-(beta-D-glucopyranosyl)-D-pantothenic acid

We analyzed beta-glucosidase-catalyzed transglucosylation to D-pantothenic acid using a reversed-phase HPLC system in order to obtain 4'-O-(beta-D-glu copyranosyl)-D-pantothenic acid (PaG) at a higher yield. The HPLC system wa s simpler and more straightforward for the PaG analysis than the previously employed bioassay method and could also be adopted for efficient isolation of PaG. Penicillium decumbens naringinase showed the highest glucosyl tran sfer activity to D-pantothenic acid, and the reaction using smaller amounts of naringinase for prolonged periods of reaction time (70 he) was importan t to attain higher yields of glucosyl transfer. Maximum overall yields of P aG of 10 and 4% (mol/mol, based on D-pantothenic acid) were obtained using beta, beta'-trehalose and cellobiose, respectively, as glucosyl donors. The value was 3.6- and 1.4-times higher, respectively, than that obtained by p revious synthesis and isolation procedures.