Comparison of University of Wisconsin, Euro-Collins, low-potassium dextran, and Krebs-Henseleit solutions for hypothermic lung preservation

Objective: We sought to test the effectiveness of 4 different solutions for hypothermic rat lung preservation. Methods: One hundred ninety-two rats were used. The rats were divided into 4 groups, and University of Wisconsin, Euro-Collins, low-potassium dextran, or Krebs-Henseleit solution was used in each group. They were further divi ded into 6 subgroups of 8 rats each. The lungs were preserved at 4 degrees C for 0, 4, 6, 8, 12, or 24 hours, respectively, and lung function was stud ied by using a living rat perfusion model. Results: Pulmonary arterial flow decreased in each group after 4 to 6 hours of preservation; the low-potassium dextran group decreased the least and t he Krebs-Henseleit group decreased the most, Pulmonary vascular resistance increased in each group after 6 hours of preservation; the Krebs-Henseleit group increased the most. Although airway pressure increased, static lung c ompliance and gas exchange capacity decreased after 8 hours of preservation ; the Krebs-Henseleit group exhibited the worst values. Lung tissue wet/dry weight ratio increased gradually during preservation; the University of Wi sconsin group exhibited the least increase. An ultrastructural study indica ted the least morphologic changes in the low-potassium dextran group at 24 hours. Conclusions: At 4 degrees C, all solutions preserved rat lungs for 4 hours with acceptable function. However, 6 hours of preservation resulted in dama ged pulmonary function in some lungs, and this damage increased when preser vation time was extended. The lungs preserved in low-potassium dextran solu tion had the best overall function, but the lungs preserved in University o f Wisconsin solution had less edema.