Sf. Chien et al., Comparison of University of Wisconsin, Euro-Collins, low-potassium dextran, and Krebs-Henseleit solutions for hypothermic lung preservation, J THOR SURG, 119(5), 2000, pp. 921-930
Citations number
35
Categorie Soggetti
Cardiovascular & Respiratory Systems","Cardiovascular & Hematology Research
Objective: We sought to test the effectiveness of 4 different solutions for
hypothermic rat lung preservation.
Methods: One hundred ninety-two rats were used. The rats were divided into
4 groups, and University of Wisconsin, Euro-Collins, low-potassium dextran,
or Krebs-Henseleit solution was used in each group. They were further divi
ded into 6 subgroups of 8 rats each. The lungs were preserved at 4 degrees
C for 0, 4, 6, 8, 12, or 24 hours, respectively, and lung function was stud
ied by using a living rat perfusion model.
Results: Pulmonary arterial flow decreased in each group after 4 to 6 hours
of preservation; the low-potassium dextran group decreased the least and t
he Krebs-Henseleit group decreased the most, Pulmonary vascular resistance
increased in each group after 6 hours of preservation; the Krebs-Henseleit
group increased the most. Although airway pressure increased, static lung c
ompliance and gas exchange capacity decreased after 8 hours of preservation
; the Krebs-Henseleit group exhibited the worst values. Lung tissue wet/dry
weight ratio increased gradually during preservation; the University of Wi
sconsin group exhibited the least increase. An ultrastructural study indica
ted the least morphologic changes in the low-potassium dextran group at 24
hours.
Conclusions: At 4 degrees C, all solutions preserved rat lungs for 4 hours
with acceptable function. However, 6 hours of preservation resulted in dama
ged pulmonary function in some lungs, and this damage increased when preser
vation time was extended. The lungs preserved in low-potassium dextran solu
tion had the best overall function, but the lungs preserved in University o
f Wisconsin solution had less edema.