K. Ishikawa et al., Difference in the responses after administration of granulocyte colony-stimulating factor in septic patients with relative neutropenia, J TRAUMA, 48(5), 2000, pp. 814-824
Objective: The objective of this study was to classify the clinical respons
es after administration of granulocyte colony-stimulating factor (G-CSF) in
septic patients with relative neutropenia.
Patients and Methods: We administered recombinant human G-CSF (2 mu g/kg) s
ubcutaneously once a day for 5 days to 30 septic patients with white cell c
ounts below 5,000 cells/mm(3). Absolute neutrophil count (ANC), neutrophil
differentiation, and serum concentration of G-CSF were determined serially.
Bone marrow also was analyzed before and after treatment.
Results: Neutrophil responses to G-CSF varied from good (ANC > 10,000/mm(3)
, group G, n = 20) to moderate (ANC < 10,000/mm(3), group M, n = 5) to poor
(no increase in ANC, group P, n = 5). Before G-CSF administration, the thr
ee groups showed no differences in ANC but did show significant differences
in serum concentration of G-CSF. G-CSF concentration was 0.16 +/- 0.03 ng/
mL in group G, 7.0 +/- 3.0 ng/mL in group M, and 270 +/- 90 ng/mL in group
P. Immature neutrophils accounted for 35.0 +/- 3.7% of peripheral leukocyte
s in group P but only 5.1 +/- 0.6% in group G. Although bone marrow was dep
ressed in all groups before C-CSF treatment, nucleated cell count increased
significantly after rhG-CSF treatment in groups G and M. Survival rate aft
er 4 weeks was 90% in group G and 100% in group M; no patient in group P su
rvived.
Conclusion: G-CSF administration was effective in septic patients with a lo
w percentage of immature neutrophils and insufficient endogenous G-CSF, It
had little effect on patients with a high percentage of immature neutrophil
s whose C-CSF production was up-regulated and whose bone marrow,vas severel
y depressed.