Treatment of sanitary-important bacteria by bacteriocin substance V24 in cattle dung water

Quantification of sanitary-important bacteria (e.g. Enterobacteriaceae), as well as indicators of environmental contamination, was assessed in samples of cattle dung from 25 cattle farms in 15 north-eastern Slovakia districts . The inhibitory effect of crude bacteriocin extract CBE V24 from Enterococ cus faecalis V24 against Listeria monocytogenes Ohio and Yersinia enterocol itica YE85 was examined in cattle dung water with the aim of finding a new way of eliminating the health risk of the animal slurry. The following bact erial groups were quantified: Salmonella spp., Shigella-like spp., Proteus spp., Enterobacter spp., Citrobacter spp., Pseudomonas spp., Escherichia co li, Listeria spp., staphylococci, streptococci and enterococci (the average count ranged from 10(2) up to 10(4) cfu ml(-1)). Antagonistic effect of th e crude bacteriocin from Enterococcus faecalis V24 in the range of 100-600 Arbitrary units per ml (AU ml(-1)) was shown against the following bacteria : Enterobacter cloacae, Ent. asburiae, Proteus spp., Salmonella spp., Acine tobacter lwoffi, L. monocytogenes as well as Y. enterocolitica YE85. During tests performed to study the inhibitory effect of the crude bacteriocin CB E V24 (concentration 800, 1600 AU ml(-1)) against L. monocytogenes Ohio and Y. enterocolitica YE85 in experimentally contaminated cattle dung, a reduc tion of 2.03 and 1.44 log cfu ml(-1), respectively, was already noted after 1 h after crude bacteriocin CBE V24 addition.