RAPD-fingerprinting of Serratia marcescens after formaldehyde inactivationof DNase activity

Authors
Polyzou, A Sofianou, D Pournaras, S Tsakris, A
Citation
A. Polyzou et al., RAPD-fingerprinting of Serratia marcescens after formaldehyde inactivationof DNase activity, LETT APPL M, 30(5), 2000, pp. 419-421
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
30
Issue
5
Year of publication
2000
Pages
419 - 421
Database
ISI
SICI code
0266-8254(200005)30:5<419:ROSMAF>2.0.ZU;2-S
Abstract
Serratia marcescens strains commonly cause hospital outbreaks. A random amp lified polymorphic DNA (RAPD) assay was applied to the epidemiology of Ser. marcescens using formaldehyde fixation and boiling of bacterial cells for DNA extraction. The method preserved bacterial DNA and gave optimal results of RAPD-fingerprinting, facilitating the investigation of hospital infecti ons caused by Ser. marcescens.