Comparison of the mycobacteria growth indicator tube with radiometric and solid culture for isolation of mycobacteria from clinical specimens and susceptibility testing of Mycobacterium tuberculosis
F. Ardito et al., Comparison of the mycobacteria growth indicator tube with radiometric and solid culture for isolation of mycobacteria from clinical specimens and susceptibility testing of Mycobacterium tuberculosis, MICROBIOLO, 23(2), 2000, pp. 151-158
We compared the mycobacteria growth indicator tube (MGIT) system with the B
ACTEC 460 TB and Loewenstein-Jensen (LJ) systems for the recovery of mycoba
cteria (acid-fast bacilli [AFB]) from 600 clinical specimens. A total of 50
AFB (32 Mycobacterium tuberculosis complex, 10 M. avium complex, 3 M. gord
onae, 3 M. xenopi, 1 M. terrae and 1 M. fortuitum) were detected. MGIT reco
vered 50 isolates of AFB (100% sensitivity), and BACTEC 460 TB and LJ recov
ered 49 (98% sensitivity) and 19 (38% sensitivity) AFB isolates, respective
ly. The mean times to detect mycobacteria were 10, 10 and 25 days for MGIT,
BACTEC 460, and LJ slants. All isolates of M. tuberculosis complex were te
sted for susceptibility to streptomycin, isoniazid, rifampin, and ethambuto
l with the MGIT and BACTEC 460 TB. Both systems yielded identical susceptib
ility data with different mean times to report (5.38 days for MGIT versus 7
.33 days for BACTEC 460 TB, P<0.05). The results suggest that MGIT is equiv
alent to BACTEC 460 TB in its ability to support the growth of mycobacteria
, but significantly more efficient than LJ. MGIT may also be used for susce
ptibility testing of primary antituberculosis drugs.