Sequence of the gene encoding an alkaline serine proteinase of Bacillus pumilus TYO-67

The complete nucleotide sequence of the gene encoding an alkaline serine pr oteinase (aprP) of Bacillus pumilus TYO-67 was determined, The sequence ana lysis showed an open reading frame of 1,149 bp (383 amino acids) that encod ed a signal peptide consisting of 29 residues and a propeptide of 79 residu es, The deduced 3 amino acid residues, D-32, H-64, and S-221, were identica l with 3 essential amino acids in the catalytic center of subtilases. The s equence around these residues revealed that APRP was a new member of the tr ue subtilisin subgroup of the subtilisin family, The highest homology was f ound in subtilisin NAT at 64.4% in the DNA sequence. The residue S-189 of A PRP was different from those of other subtilases.