Genetic variation among European strains of Saccharomyces paradoxus: Results from DNA fingerprinting

We used microsatellite fingerprinting and RAPD analysis to characterize 28 wild European strains of Saccharomyces paradoxus. In contrast to our result s from a previous allozyme survey [NAUMOV et al. Int. J. Syst. Bacteriol. 4 7: 341-344 (1997a)], these methods revealed extensive genetic variation. Th e RAPD primers 5'AATCGGGCTG and 5'GGGTAACGCC and the microsatellite primer (GTG)(5) yielded reproducible amplification patterns of sufficient clarity and variability to distinguish individual strains from the wild. UPGMA anal ysis tended to group the strains according to climatic and geographic origi n. A comparative study of Ty1 sequence having multiple chromosomal location was also done. Each wild 5. paradoxus isolate shows a unique hybridization patten allowing discrimination to the strain level.