Polyphasic identification of wild yeast strains isolated from Greek sourdoughs

A total of forty-five wild yeast strains were isolated from five traditiona l Greek wheat sourdoughs. Strains were identified using the classical ident ification technique along with the sodium dodecyl sulfate-polyacrylamide ge l electrophoresis of whole cell proteins (SDS-PAGE), Fourier transform-infr ared spectroscopy (FT-IR) and the randomly amplified polymorphic DNA-polyme rase chain reaction analysis (RAPD-PCR). The latter methods confirmed the c lassical identification. According to the results obtained, fourteen strain s were identified as Saccharomyces cerevisiae strains, twenty-five as Pichi a membranaefaciens strains and six as Yarrowia lipolytica.