Cloning and characterization of the murine coagulation factor X gene

gene encoding murine coagulation factor X (fX) was isolated and characteriz ed from a lambda FIX II library generated from murine genomic DNA. The 2013 0 bp sequence contains 18049 nucleotides that extend from the initiating me thionine to the polyadenylation site. 1056 nucleotides 5' of the start codo n were determined and contain putative start sites for the FX mRNA as well as sites for binding of putative transcription factors. The sequence extend s 1024 3' of the polyadenylattion site.The gene contains 8 exons and 7 intr ons which were determined by comparing the mouse FX cDNA and gene sequences . The exonic structure of the gene is similar to that of the other mammalia n vitamin K-dependent serine proteases of the coagulation system. These inc lude an exon encoding the prepropepetide, the gla-domain, a short helical s tack, two exons for the two EGF domains, the activation pepetide, and two e xons encoding the serine protease domain. The 5' sequence of the mouse FX g ene overlaps with the 3' region of the FVII gene indicating that the murine FVII and FX gene are arranged in a head to tail arrangement as they are in humans.