IgE-reactivity to major Japanese cedar (Cryptomeria japonica) pollen allergens (Cry j 1 and Cry j 2) by ELISA in dogs with atopic dermatitis

The present study investigated IgE-reactivity to two major Japanese cedar ( Cryptomeria japonica, C. japonica) pollen allergens (Cry j 1 and Cry j 2) i n dogs with atopic dermatitis by use of a fluorometric ELISA. The serum sam ples from 27 dogs that showed IgE-sensitivity to crude C. japonica pollen a llergen by ELISA were tested for specific IgE to the two major allergens. A ll 27 dogs had anti-Cry j 1 IgE, and 10 (37%) had anti-Cry j 2 IgE. Inhibit ion of binding of dog specific IgE to crude C. japonica pollen allergen was carried out by addition of Cry j 1. When serum samples containing anti-Cry j 1 IgE but no anti-Cry j 2 IgE were incubated with Cry j 1, specific IgE binding to crude C. japonica pollen allergen was almost abolished. These fi ndings suggest that Cry j 1 is a major allergen in dogs. (C) 2000 Elsevier Science B.V. All rights reserved.